Internalization and degradation of natriuretic peptide receptor-A is stimulated by ligand binding

نویسندگان

  • Darcy R Flora
  • Sean D Conner
  • Lincoln R Potter
چکیده

Background Natriuretic peptide receptor-A (NPR-A) is a transmembrane receptor guanylyl cyclase that binds and mediates the effects of atrial and B-type natriuretic peptides (ANP/ BNP). Internalization and ligand-dependent degradation of NPR-A is controversial, in part due to the use of ligand binding studies to predict the cellular location of the receptor. Here, we used a more direct sequential immunoprecipitation-western blot assay to demonstrate that longterm ANP exposure increases NPR-A degradation in primary, immortalized, and transfected cells.

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Correction to "Antibody Tracking Demonstrates Cell Type-Specific and Ligand-Independent Internalization of Guanylyl Cyclase A and Natriuretic Peptide Receptor C".

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عنوان ژورنال:

دوره 9  شماره 

صفحات  -

تاریخ انتشار 2009